Laboratory diagnostics in hematopoietic peripheral stem cell transplantation

Abstract:

Transplantation of CD34+ hematopoietic stem cells is nowadays a routine method in the treatment of lymphomas, leukemias, myelodysplastic and myeloproliferative syndromes, as well as immunodeficiency and hemoglobinopathy. The method of immunophenotyping by flow cytometry is a state of the art method for determining CD34+ hematopoietic stem cells and is indispensable for assessing the adequacy of the transplant. It is based on the multiparametric definition of CD34+ hematopoietic stem cells based on their light scattering characteristics (FSC x SSC) and weak expression of CD45 antigen. Its application enables reliable and fast (within 2 hours) determination of CD34+ hematopoietic stem cells in peripheral blood, concentrate of mobilized CD34+ hematopoietic stem cells of peripheral blood (leukapheresis product), bone marrow and umbilical blood.

Key words:
CD34+ hematopoietic stem cells; flow cytometer DxFlex; flow cytometry; ISHAGE protocol